HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
![HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)](https://www.abcam.com/ps/products/197/ab197609/Images/ab197609-238354-anti-brd4-antibody-epr51502-hrp-western-blot.jpg "HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5150(2)] to Brd4
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Brd4 antibody [EPR5150(2)]
See all Brd4 primary antibodies -
Description
HRP Rabbit monoclonal [EPR5150(2)] to Brd4 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, CaCo2, RAW264.7 and NIH3T3 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 3.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR5150(2) -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)All lanes : HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 152 kDa
Exposure time: 2 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197609 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)](https://www.abcam.com/ps/products/197/ab197609/Images/ab197609-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
