Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-Brd4 antibody [BL-149-2H5] (ab243862)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-149-2H5] to Brd4
Suitable for: WB, ChIP-sequencing, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-Brd4 antibody [BL-149-2H5]
See all Brd4 primary antibodies
Description

Rabbit monoclonal [BL-149-2H5] to Brd4
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP-seq	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Brd4 aa 1312-1362. The exact sequence is proprietary. NP_490597.1 and Gene ID 23476.
Database link: O60885
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-149-2H5
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] (ab243862)

Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] (ab243862)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] (ab243862)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for brd4 using ab243862 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] (ab243862)

brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with ab243862 at 20 µL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.
Lane 1: ab243862 IP in HeLa cell lysate.

Lane 2: Contol IgG in HeLa cell lysate.
Western blot - Anti-Brd4 antibody [BL-149-2H5] (ab243862)

Western blot analysis using ab243862 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate.
Lane 2: HEK-293T whole cell lysate.
Lane3: Jurkat whole cell lysate.
Lane 4: MB-231 whole cell lysate.
Lane 5: HepG2 whole cell lysate.
Lane 6: A549 whole cell lysate.
Lane 7: SW620 whole cell lysate.
Lane 8: SK whole cell lysate.
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
Anti-Brd4 antibody [BL-149-2H5] (ab243862)

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