Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12960] to BRD1/BRL - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free
    See all BRD1/BRL primary antibodies

  • Description

    Rabbit monoclonal [EPR12960] to BRD1/BRL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562 and Raji cell lysate; IHC-P: Human testis and lung carcinoma tissue, Mouse testis tissue and Rat testis tissue. ICC: K-562 and F9 cell line.

  • General notes

    ab250317 is the carrier-free version of ab181060 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250317 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BRD1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Immunocytochemistry - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)

    Immunocytochemistry analysis of F9 cell line labelling BRD1/BRL with ab181060 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. . Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

    Confocal image showing no staining in F9 cell line

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    This data was developed using ab181060, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunocytochemistry - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Immunocytochemistry - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)

    Immunocytochemistry analysis of K-562 cell line labelling BRD1/BRL with ab181060 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. . Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

    Confocal image showing no staining in K-562 cell line

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    This data was developed using ab181060, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    This data was developed using ab181060, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human lung carcinoma. The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    This data was developed using ab181060, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 10602503). The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
  • Western blot - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Western blot - Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    All lanes : Anti-BRD1/BRL antibody [EPR12960] (ab181060) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 120 kDa



    This data was developed using ab181060, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
    Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)

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