Anti-BRD1/BRL antibody (ab71877)
Key features and details
- Rabbit polyclonal to BRD1/BRL
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRD1/BRL antibody
See all BRD1/BRL primary antibodies -
Description
Rabbit polyclonal to BRD1/BRL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human BRD1/BRL aa 150-250 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab71876) -
General notes
This product was previously labelled as BRD1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab71877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB ICC/IF Application notesICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 137 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 417731 Chicken
- Entrez Gene: 23774 Human
- Entrez Gene: 223770 Mouse
- Omim: 604589 Human
- SwissProt: O95696 Human
- Unigene: 127950 Human
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Alternative names
- BR140-like protein antibody
- BRD1 antibody
- BRD1_HUMAN antibody
see all
Images
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Western blot - Anti-BRD1/BRL antibody (ab71877)All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 :Raji whole cell lysate (ab30124) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-BRD1/BRL antibody (ab71877)ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml. -
Western blot - Anti-BRD1/BRL antibody (ab71877)This image is a courtesy of Antibody Solutions LtdAll lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : Lysates prepared from Mouse testis tissue.
Lane 2 : Lysates prepared from Mouse Lung tissue.
Lane 3 : Lysates prepared from Mouse Kidney tissue.
Lane 4 : Lysates prepared from GC-1 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 92 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 7 minutesGC-1 - mouse spermatogonia cell line
Protocols
Datasheets and documents
References (2)
ab71877 has been referenced in 2 publications.
- Wu Q et al. A chemical toolbox for the study of bromodomains and epigenetic signaling. Nat Commun 10:1915 (2019). PubMed: 31015424
- Kang H et al. Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in Drosophila. Genes Dev 31:1988-2002 (2017). PubMed: 29070704
Images
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Western blot - Anti-BRD1/BRL antibody (ab71877)All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 :Raji whole cell lysate (ab30124) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-BRD1/BRL antibody (ab71877)ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
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Western blot - Anti-BRD1/BRL antibody (ab71877) This image is a courtesy of Antibody Solutions LtdAll lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : Lysates prepared from Mouse testis tissue.
Lane 2 : Lysates prepared from Mouse Lung tissue.
Lane 3 : Lysates prepared from Mouse Kidney tissue.
Lane 4 : Lysates prepared from GC-1 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 92 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 7 minutesGC-1 - mouse spermatogonia cell line
