Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BRD9 antibody [EPR23888-5] (ab259839)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23888-5] to BRD9
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-BRD9 antibody [EPR23888-5]
See all BRD9 primary antibodies
Description

Rabbit monoclonal [EPR23888-5] to BRD9
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Rat

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, 293T, NIH/3T3, PC-12, K-562, HDLM-2, LNCaP, Jurkat, Neuro-2a and RAW 264.7, C6 lysates. IHC-P: Human testis, Mouse testis, Rat testis tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Rat
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23888-5
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

All lanes : Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : HDLM-2 (human hodgkin lymphoma ) whole cell lysate
Lane 3 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
Lane 4 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 80 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBSTLysates used in this blot have experienced freeze-thaw cycles.

Exposure time: Lanes 1-2: 37 seconds;Lanes 3-4: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on human testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

All lanes : Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

Lanes 1 & 5 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lanes 4 & 6 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 80 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID:32457312; 31015438 ).

Lysates were made freshly and used in WB immediately to minimize protein degradation (lane1-4). Lanes 5-6 are the lysates from same cell lines but have experienced freeze-thaw cycles.

Exposure time: Lanes 1-4: 37 seconds; Lanes 5-6: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

All lanes : Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Observed band size: 80 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lysates used in this blot have experienced freeze-thaw cycles.

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-BRD9 antibody [EPR23888-5] (ab259839)

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