Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [CL1115] to Brd4
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-Brd4 antibody [CL1115]
See all Brd4 primary antibodies
Description

Mouse monoclonal [CL1115] to Brd4
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Brd4 aa 1000-1200.
Database link: O60885

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptidesequence PQPAKPQQVIQHHHSasdetermined by overlapping synthetic peptides.
Positive control
- IHC-P: Human stomach cancer, testis, cerebral cortex and kidney tissues. WB: U-251 MG cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL1115
Isotype

IgG1
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [CL1115] (ab244221)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling BRD4 using ab244221 at 10 µg/ml, showing specific staining in the nucleoplasm in green. Microtubles are visualized in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

Paraffin-embedded human stomach cancer tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-Brd4 antibody [CL1115] (ab244221)

All lanes : Anti-Brd4 antibody [CL1115] (ab244221) at 1 µg/ml

Lane 1 : U-251 MG (human brain glioma cell line) cell lysate transfected with control siRNA
Lane 2 : U-251 MG cell lysate transfected with target specific siRNA probe #1
Lane 3 : U-251 MG cell lysate transfected with target specific siRNA probe #2

Predicted band size: 152 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

Paraffin-embedded human testis tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-Brd4 antibody [CL1115] (ab244221)

Anti-Brd4 antibody [CL1115] (ab244221) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 152 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

Paraffin-embedded human cerebral cortex tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

Paraffin-embedded human kidney tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com