Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR7 antibody [EPR23192-57] (ab253187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23192-57] to CCR7
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CCR7 antibody [EPR23192-57]
    See all CCR7 primary antibodies

  • Description

    Rabbit monoclonal [EPR23192-57] to CCR7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, Human Hodgkin's lymphoma, Mouse spleen and Rat spleen tissues. IHC-Fr: Mouse spleen and Rat spleen tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling CCR7 with ab253187 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: No staining on mouse pancreas  is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human pancreas. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat spleen. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on RS cells in human Hodgkin's lymphoma. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human tonsil. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Anti-CCR7 antibody [EPR23192-57] (ab253187)
    Anti-CCR7 antibody [EPR23192-57] (ab253187)

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