Anti-CD28 antibody [EPR22311-17] (ab242124)
![Anti-CD28 antibody [EPR22311-17] (ab242124)](https://www.abcam.com/ps/products/242/ab242124/Images/ab242124-330833-anti-cd28-antibody-epr22311-17-immunohistochemistry.jpg "Anti-CD28 antibody [EPR22311-17] (ab242124)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22311-17] to CD28
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse
Overview
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Product name
Anti-CD28 antibody [EPR22311-17]
See all CD28 primary antibodies -
Description
Rabbit monoclonal [EPR22311-17] to CD28 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse thymus tissue lysate; EL4.IL-2 whole cell lysate. IHC-P: Mouse spleen tissue. IP: EL4.IL-2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22311-17 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody [EPR22311-17] (ab242124)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue stained for CD28 using ab242124 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in periarterial lymphatic sheath of mouse spleen (PMID: 7489738). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
The section was incubated with ab242124 for 30 mins at 37℃. Immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CD28 antibody [EPR22311-17] (ab242124)](https://www.abcam.com/ps/products/242/ab242124/Images/ab242124-330832-anti-cd28-antibody-epr22311-17-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD28 antibody [EPR22311-17] (ab242124)CD28 was immunoprecipitated from 0.35 mg EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate with ab242124 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab242124 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: EL4.IL-2 whole cell lysate 10 µg (input).
Lane 2: ab242124 IP in EL4.IL-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242124 in EL4.IL-2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds. -
![Western blot - Anti-CD28 antibody [EPR22311-17] (ab242124)](https://www.abcam.com/ps/products/242/ab242124/Images/ab242124-330831-anti-cd28-antibody-epr22311-17-western-blot.jpg)
Western blot - Anti-CD28 antibody [EPR22311-17] (ab242124)All lanes : Anti-CD28 antibody [EPR22311-17] (ab242124) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : EL4.IL-2 (mouse lymphoma t lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 40-50,80 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Bands between 40-50 kDa are likely to be glycosylated forms (PMID: 7512728), whereas the 80 kDa band represents the dimer (PMID: 2157764).
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![Anti-CD28 antibody [EPR22311-17] (ab242124)](https://www.abcam.com/ps/products/242/ab242124/Images/ab242124-5-benefits-of-recombinant-antibodies.png)
Anti-CD28 antibody [EPR22311-17] (ab242124)
