Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR083G] to CD27 - BSA free
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CD27 antibody [BLR083G] - BSA free
    See all CD27 primary antibodies

  • Description

    Rabbit monoclonal [BLR083G] to CD27 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD27 aa 1-200 (extracellular). NP_001233.1
    Database link: P26842

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil tissue. ICC: Ramos cells. IP: Ramos whole cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272072 is the carrier-free version of ab265589. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265589).

  • Immunocytochemistry - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)
    Immunocytochemistry - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

    Immunocytochemical analysis of formalin-fixed, paraffin-embedded ramos cells labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265589).

  • Immunoprecipitation - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)
    Immunoprecipitation - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

    CD27 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265589 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265589 at 1/1000 dilution.


    Lane 1: ab265589 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG.

    Exposure time: 10 secs.

    Cells prepared using NETN lysis buffer.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265589).

  • Anti-CD27 antibody [BLR083G] - BSA free (ab272072)
    Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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