Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR083G] to CD27 - BSA free
Suitable for: WB, IP, IHC-P, ICC
Reacts with: Human

Product name

Anti-CD27 antibody [BLR083G] - BSA free
See all CD27 primary antibodies
Description

Rabbit monoclonal [BLR083G] to CD27 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD27 aa 1-200 (extracellular). NP_001233.1
Database link: P26842

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human tonsil tissue. ICC: Ramos cells. IP: Ramos whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272072 is the carrier-free version of ab265589. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR083G
Isotype

IgG
Research areas
- Cancer
- Tumor immunology
- Cytokines
- TNF

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265589).
Immunocytochemistry - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded ramos cells labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265589).
Immunoprecipitation - Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

CD27 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265589 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265589 at 1/1000 dilution.

Lane 1: ab265589 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.

Exposure time: 10 secs.

Cells prepared using NETN lysis buffer.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265589).
Anti-CD27 antibody [BLR083G] - BSA free (ab272072)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com