Anti-CD27 antibody [EPR8568] (ab133761)
![Anti-CD27 antibody [EPR8568] (ab133761)](https://www.abcam.com/ps/products/133/ab133761/Images/ab133761-147874-anti-cd27-antibody-epr8568-western-blot.jpg "Anti-CD27 antibody [EPR8568] (ab133761)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8568] to CD27
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CD27 antibody [EPR8568]
See all CD27 primary antibodies -
Description
Rabbit monoclonal [EPR8568] to CD27 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within the extracellular domain of Human CD27 (UniProt ID: P26842).
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Positive control
- Ramos cells and cell lysate
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 7.60 x 10 -13 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8568 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD27 antibody [EPR8568] (ab133761)Anti-CD27 antibody [EPR8568] (ab133761) at 1/1000 dilution + Ramos cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 29 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8568] (ab133761)](https://www.abcam.com/ps/products/133/ab133761/Images/ab133761-147873-anti-cd27-antibody-epr8568-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8568] (ab133761)Immunofluorescent staining of CD27 in Ramos cells, using ab133761 at a 1/50 dilution. -
![OI-RD Scanning - Anti-CD27 antibody [EPR8568] (ab133761)](https://www.abcam.com/ps/products/133/ab133761/Images/ab133761-147872-anti-cd27-antibody-epr8568.jpg)
OI-RD Scanning - Anti-CD27 antibody [EPR8568] (ab133761) -
![Anti-CD27 antibody [EPR8568] (ab133761)](https://www.abcam.com/ps/products/133/ab133761/Images/ab133761-4-benefits-of-recombinant-antibodies.png)
Anti-CD27 antibody [EPR8568] (ab133761)
