Mouse IL-6 R alpha Matched Antibody Pair Kit (ab213462)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 23 pg/ml - 1500 pg/ml
Overview
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Product name
Mouse IL-6 R alpha Matched Antibody Pair Kit
See all IL-6R kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.33 pg/ml -
Range
23 pg/ml - 1500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-6 R alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-6 R alpha.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab203360. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse IL-6R alpha Capture Antibody 2 x 50µg 1 x 50µg Mouse IL-6R alpha Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse IL-6R alpha Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
Tissue specificity
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
Post-translational
modificationsA short soluble form may also be released from the membrane by proteolysis. -
Cellular localization
Secreted and Basolateral cell membrane. - Information by UniProt
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Alternative names
- CD 126
- CD126
- CD126 antigen
see all -
Database links
- Entrez Gene: 16194 Mouse
- SwissProt: P22272 Mouse
- Unigene: 2856 Mouse
Images
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Mouse IL-6 R alpha standard curve.Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse IL-6 R alpha Matched Antibody Pair Kit (ab213462)To learn more about the advantages of recombinant antibodies see here.
