Anti-CD27 antibody [BLR083G] (ab265589)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR083G] to CD27
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-CD27 antibody [BLR083G]
See all CD27 primary antibodies -
Description
Rabbit monoclonal [BLR083G] to CD27 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human CD27 aa 1-200 (extracellular). NP_001233.1
Database link: P26842 -
Positive control
- IHC-P: Human tonsil tissue. ICC: Ramos cells. WB: Ramos, CCRF-CEM and Daudi whole cell lysate. IP: Ramos whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BLR083G -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CD27 antibody [BLR083G] (ab265589) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Ramos whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : Raji whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : U-2 OS whole cell lysate
Lane 7 : Daudi whole cell lysate
Lane 8 : MCF7 whole cell lysate
Lane 9 : CCRF-CEM whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG
Predicted band size: 29 kDa
Exposure time: 3 minutesCells prepared using NETN lysis buffer.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
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Immunocytochemical analysis of formalin-fixed, paraffin-embedded ramos cells labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
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CD27 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265589 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265589 at 1/1000 dilution.
Lane 1: ab265589 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
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