Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR083G] to CD27
Suitable for: WB, IP, IHC-P, ICC
Reacts with: Human

Product name

Anti-CD27 antibody [BLR083G]
See all CD27 primary antibodies
Description

Rabbit monoclonal [BLR083G] to CD27
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD27 aa 1-200 (extracellular). NP_001233.1
Database link: P26842

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human tonsil tissue. ICC: Ramos cells. WB: Ramos, CCRF-CEM and Daudi whole cell lysate. IP: Ramos whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR083G
Isotype

IgG
Research areas
- Cancer
- Tumor immunology
- Cytokines
- TNF

Western blot - Anti-CD27 antibody (ab265589)

All lanes : Anti-CD27 antibody [BLR083G] (ab265589) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : Ramos whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : Raji whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : U-2 OS whole cell lysate
Lane 7 : Daudi whole cell lysate
Lane 8 : MCF7 whole cell lysate
Lane 9 : CCRF-CEM whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit IgG

Predicted band size: 29 kDa

Exposure time: 3 minutes

Cells prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody (ab265589)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
Immunocytochemistry - Anti-CD27 antibody (ab265589)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded ramos cells labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
Immunoprecipitation - Anti-CD27 antibody (ab265589)

CD27 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265589 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265589 at 1/1000 dilution.
Lane 1: ab265589 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.

Exposure time: 10 secs.

Cells prepared using NETN lysis buffer.
Anti-CD27 antibody [BLR083G] (ab265589)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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