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Immunology Adaptive Immunity T Cells CD

Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [LPFS2/1611] to CD27 - BSA and Azide free
  • Suitable for: IHC-P, Protein Array, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free
    See all CD27 primary antibodies
  • Description

    Mouse monoclonal [LPFS2/1611] to CD27 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Protein Array, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human CD27 aa 1-260. The exact sequence is proprietary.
    Database link: P26842

  • Positive control

    • ICC/IF: Ramos cells. IHC-P: Human tonsil, spleen and colon tissue. Flow Cyt: Ramos cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    LPFS2/1611
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Tumor immunology
    • Cytokines
    • TNF
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

  • Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Immunocytochemistry/ Immunofluorescence staining of Ramos (Human Burkitt's lymphoma cell line) cells staind for CD27 using ab268144 at 2µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

  • Flow Cytometry - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Flow Cytometry - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Flow cytometric analysis of Ramos (Human Burkitt's lymphoma cell line) cells labeling CD27 using ab268144 at 2 μg/million cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

  • Protein Array - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Protein Array - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Protein Array containing more than 19,000 full-length human proteins using ab268144.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (ab268268)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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