Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20539] to CD272/BTLA - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free
    See all CD272/BTLA primary antibodies

  • Description

    Rabbit monoclonal [EPR20539] to CD272/BTLA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A20 cells.

  • General notes

    ab224676 is the carrier-free version of ab212089 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab224676 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CD272

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20539

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)
    Flow Cytometry - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)

    Flow cytometric analysis of mouse splenocytes labeling CD272/BTLA with ab212089 at 1/500 dilution (right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Mouse splenocytes stained with CD3 and ab212089. Total viable cells were gated for the FC image. T cells (CD3+, upper population) show a moderate positive signal while B cells (lower right population in right panel) show stronger positive signal. The expression pattern is also reported in literature (PMID: 19949073).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212089).

  • Flow Cytometry - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)
    Flow Cytometry - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)

    Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD272/BTLA with ab212089 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Total viable cells were gated for the FC image.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212089).

  • Immunoprecipitation - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)
    Immunoprecipitation - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)

    CD272/BTLA was immunoprecipitated from 0.35 mg mouse spleen lysate with ab212089 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212089 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.

    Lane 1: Mouse spleen lysate  10 μg (Input).

    Lane 2: ab212089 IP in mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab212089 in mouse spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212089).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)
    Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)

    Immunofluorescent analysis of 100% methanol fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD272/BTLA with ab212089 at 1/100 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on A20 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212089).

  • Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)
    Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (ab224676)

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