Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24537-15] to NOX2/gp91phox - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free
    See all NOX2/gp91phox primary antibodies

  • Description

    Rabbit monoclonal [EPR24537-15] to NOX2/gp91phox - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver, colon and spleen tissues.

  • General notes

    ab280959 is the carrier-free version of ab280952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)

    This data was developed using ab280952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling NOX2/gp91phox with ab280952 at 1/500 (1.06 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Kuffer cells of human liver. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)

    This data was developed using ab280952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling NOX2/gp91phox with ab280952 at 1/500 (1.06 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human colon. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)

    This data was developed using ab280952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NOX2/gp91phox with ab280952 at 1/500 (1.06 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
    Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)

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