Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
![Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)](https://www.abcam.com/ps/products/280/ab280959/Images/ab280959-2-anti-nox2gp91phox-antibody-epr2453715-immunohistochemistry-human-liver.jpg "Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24537-15] to NOX2/gp91phox - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free
See all NOX2/gp91phox primary antibodies -
Description
Rabbit monoclonal [EPR24537-15] to NOX2/gp91phox - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver, colon and spleen tissues.
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General notes
ab280959 is the carrier-free version of ab280952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24537-15 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
This data was developed using ab280952, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling NOX2/gp91phox with ab280952 at 1/500 (1.06 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Kuffer cells of human liver. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)](https://www.abcam.com/ps/products/280/ab280959/Images/ab280959-3-anti-nox2gp91phox-antibody-epr2453715-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)This data was developed using ab280952, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling NOX2/gp91phox with ab280952 at 1/500 (1.06 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human colon. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)](https://www.abcam.com/ps/products/280/ab280959/Images/ab280959-4-anti-nox2gp91phox-antibody-epr2453715-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)This data was developed using ab280952, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NOX2/gp91phox with ab280952 at 1/500 (1.06 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)](https://www.abcam.com/ps/products/280/ab280959/Images/ab280959-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-NOX2/gp91phox antibody [EPR24537-15] - BSA and Azide free (ab280959)
