Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20325] to Aquaporin 1
Suitable for: IHC-P, Flow Cyt, IP, ICC/IF, WB
Reacts with: Human

Product name

Anti-Aquaporin 1 antibody [EPR20325]
See all Aquaporin 1 primary antibodies
Description

Rabbit monoclonal [EPR20325] to Aquaporin 1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal kidney lysate; whole cell lysate from HEK-293T cells transfected with a GFP-tagged human Aquaporin 1 construct IHC-P: Human pancreas, kidney and cardiac muscle tissues. ICC/IF: HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct. Flow Cyt: HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct. IP: Whole cell lysate from HEK-293T transfected with a GFP-tagged human Aquaporin 1 construct.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20325
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on centroacinar cells of human pancreas is observed [PMID: 12801959].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged Aquaporin 1 expression construct or a GFP only control, labeling Aquaporin 1 with ab219055 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 647) (ab150079) secondary antibody at 1/1000 dilution.

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 647) (ab150079) at 1/1000 dilution.
Flow Cytometry - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Aquaporin 1 construct cells labeling Aquaporin 1 with ab219055 at 1/700 dilution (right). A rabbit monoclonal IgG (ab172730) was used as isotype control (left). Goat anti rabbit IgG (Alexa Fluor^® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

Quadrant markers were set based on control antibody staining (ab172730) and GFP expression. Only GFP positive population (Q2 of right panel) give Aquaporin 1 positive staining.
Western blot - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Anti-Aquaporin 1 antibody [EPR20325] (ab219055) at 1/1000 dilution + Human fetal kidney lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Aquaporin 1 was immunoprecipitated from 0.35 mg of whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Auaporin 1 construct, with ab219055 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab219055 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct, 10 µg (Input).

Lane 2: ab219055 IP in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219055 in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Western blot - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

All lanes : Anti-Aquaporin 1 antibody [EPR20325] (ab219055) at 1/1000 dilution

Lane 1 : Whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with an empty expression vector
Lane 2 : Whole cell lysate from HEK-293T transfected with a GFP-tagged human Aquaporin 1 construct

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 28 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?

Exposure time: 2 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on proximal tubules of human kidney is observed [PMID: 14592814].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on vessels of human cardiac muscle is observed [PMID: 14592814].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Aquaporin 1 antibody [EPR20325] (ab219055)

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