Anti-Aquaporin 1 antibody (ab65837)
Key features and details
- Rabbit polyclonal to Aquaporin 1
- Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Aquaporin 1 antibody
See all Aquaporin 1 primary antibodies -
Description
Rabbit polyclonal to Aquaporin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Aquaporin 1 (C terminal).
Database link: P29972 -
Positive control
- WB: Rat Kidney and lung tissue lysate, SMMC cell Lysate. Flow Cyt (Intra): U20S cells. IHC-P: Rat Kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2% Trehalose -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody (ab65837)
Aquaporin 1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Western blot - Anti-Aquaporin 1 antibody (ab65837)All lanes : Anti-Aquaporin 1 antibody (ab65837) at 0.5 µg/ml
Lane 1 : Rat Kidney Tissue Lysate with 5% Non-fat Milk/ TBS
Lane 2 : Rat LungTissue Lysate with 5% Non-fat Milk/ TBS
Lane 3 : SMMC Cell Lysate with 5% Non-fat Milk/ TBS
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 29 kDa
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Flow Cytometry - Anti-Aquaporin 1 antibody (ab65837)Overlay histogram showing U20S cells stained with ab65837 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 1 Antibody (ab65837,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody (ab65837)Aquaporin 1 was detected in frozen section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody (ab65837)Aquaporin 1 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
