Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20325] to Aquaporin 1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free
    See all Aquaporin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20325] to Aquaporin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue.

  • General notes

    ab232399 is the carrier-free version of ab219055.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on proximal tubules of human kidney is observed [PMID: 14592814]. 

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on vessels of human cardiac muscle is observed [PMID: 14592814]. 

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged Aquaporin 1 expression construct or a GFP only control, labeling Aquaporin 1 with ab219055 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.

    Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 647) (ab150079) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

  • Flow Cytometry - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Flow Cytometry - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Aquaporin 1 construct cells labeling Aquaporin 1 with ab219055 at 1/700 dilution (right). A rabbit monoclonal IgG (ab172730) was used as isotype control (left). Goat anti rabbit IgG (Alexa Fluor® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

    Quadrant markers were set based on control antibody staining (ab172730) and GFP expression. Only GFP positive population (Q2 of right panel) give Aquaporin 1 positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

  • Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Aquaporin 1 was immunoprecipitated from 0.35 mg of whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Auaporin 1 construct, with ab219055 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab219055 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct, 10 µg (Input).

    Lane 2: ab219055 IP in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219055 in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on centroacinar cells of human pancreas is observed [PMID: 12801959].  Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)
    Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

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