Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14211(B)] to D4 GDI - BSA and Azide free
  • Suitable for: ICC, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free
    See all D4 GDI primary antibodies

  • Description

    Rabbit monoclonal [EPR14211(B)] to D4 GDI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250472 is the carrier-free version of ab181259.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Western blot - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/10000 dilution + Human bone marrow tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 23 kDa



    This data was developed using ab181259, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    This data was developed using ab181259, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181259 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Immunocytochemistry - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Immunocytochemistry - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    This data was developed using ab181259, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HL60 cells (-20°Acetone-fixed) labeling D4 GDI with ab181259 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    This data was developed using ab181259, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling D4 GDI with ab181259 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Western blot - Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    All lanes : Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HL60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa



    This data was developed using ab181259, the same antibody clone in a different buffer formulation.

     

  • Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)
    Anti-D4 GDI antibody [EPR14211(B)] - BSA and Azide free (ab250472)

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