Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19819] to PDPK1 (phospho S396) - BSA and Azide free
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free
    See all PDPK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19819] to PDPK1 (phospho S396) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Recombinant fragment
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251452 is the carrier-free version of ab206341. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251452 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)
    Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)
    All lanes : Anti-PDPK1 (phospho S396) antibody [EPR19819] (ab206341) at 1/5000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with PDPK1 S396A mutant
    Lane 2 : HEK-293T whole cell lysate transfected with PDPK1 (WT)
    Lane 3 : HEK-293T whole cell lysate transfected with PDPK1 (WT), then treated with alkaline phosphatase for 1 hour

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa
    Observed band size: 60,63 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab206341, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

    Based on sequence alignment, this antigen can recognize 5 isoforms, the predicted MW are 63kDa, 58 kDa, 60 kDa, 48 kDa and 51kDa, respectively.

  • Dot Blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)
    Dot Blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)
    This data was developed using ab206341, the same antibody clone in a different buffer formulation. Dot blot analysis of PDPK1 (phospho S396) labeled with ab206341 at 1/1000 dilution. Lane 1: PDPK1 (phospho S393) peptide (aa391-401) Lane 2: PDPK1 (phospho S393) peptide (aa386-396) Lane 3: PDPK1 non-phospho peptide (aa392-402) Lane 4: PDPK1 (phospho S396) peptide (aa392-401) Lane 5: PDPK1 (phospho S396) peptide(aa393-402) Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated (ab97051) at 1/20000 dilution was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Immunoprecipitation - Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)
    Immunoprecipitation - Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)

    This data was developed using ab206341, the same antibody clone in a different buffer formulation.

    PDPK1 (phospho S396) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, transfected with PDPK1, with ab206341 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206341 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with PDPK1 whole cell lysate, 10 µg (Input).

    Lane 2: ab206341 IP in HEK-293T transfected with PDPK1 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206341 in HEK-293T transfected with PDPK1 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

  • Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)
    Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free (ab251452)

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