Anti-Slingshot homolog 1/SSH1 antibody (ab107799)
Key features and details
- Rabbit polyclonal to Slingshot homolog 1/SSH1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Slingshot homolog 1/SSH1 antibody
See all Slingshot homolog 1/SSH1 primary antibodies -
Description
Rabbit polyclonal to Slingshot homolog 1/SSH1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Mouse Slingshot homolog 1/SSH1 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab123824) -
Positive control
- This antibody gave a positive signal in both Mouse Brain and Heart tissue lysates as well as A431 and HepG2 whole cell lysates.
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General notes
This product was previously labelled as Slingshot homolog 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab107799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications RatApplication Abreviews Notes WB (4) Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 115 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 115 kDa).Target
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Function
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsPhosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 54434 Human
- Entrez Gene: 231637 Mouse
- Entrez Gene: 304580 Rat
- Omim: 606778 Human
- SwissProt: Q8WYL5 Human
- SwissProt: Q76I79 Mouse
- Unigene: 199763 Human
- Unigene: 389682 Mouse
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Alternative names
- AW551225 antibody
- Coro1c antibody
- coronin, actin binding protein 1C antibody
see all
Images
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Western blot - Anti-Slingshot homolog 1/SSH1 antibody (ab107799)All lanes : Anti-Slingshot homolog 1/SSH1 antibody (ab107799) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThe predicted molecular weight of Slingshot homolog 1/SSH1 is 115 kDa (SwissProt), however we expect to observe a banding pattern around 150 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (1)
ab107799 has been referenced in 1 publication.
- Pang D et al. Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-?B pathway. Biol Open 9:N/A (2020). PubMed: 31988091
Images
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Western blot - Anti-Slingshot homolog 1/SSH1 antibody (ab107799)All lanes : Anti-Slingshot homolog 1/SSH1 antibody (ab107799) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThe predicted molecular weight of Slingshot homolog 1/SSH1 is 115 kDa (SwissProt), however we expect to observe a banding pattern around 150 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
