Anti-Slingshot homolog 1/SSH1 antibody (ab76943)
Key features and details
- Rabbit polyclonal to Slingshot homolog 1/SSH1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Slingshot homolog 1/SSH1 antibody
See all Slingshot homolog 1/SSH1 primary antibodies -
Description
Rabbit polyclonal to Slingshot homolog 1/SSH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Slingshot homolog 1/SSH1 aa 675-725.
Database link: NP_061857.2 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
This product was previously labelled as Slingshot homolog 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76943 was affinity purified using an epitope specific to Slingshot homolog 1/SSH1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Slingshot homolog 1/SSH1 antibody (ab76943)All lanes : Anti-Slingshot homolog 1/SSH1 antibody (ab76943) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 116 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (ab76943)ab76943 at 1µg/ml staining Slingshot homolog 1/SSH1 in HeLa whole cell lysate immunoprecipitated using ab76943 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
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Immunocytochemistry/ Immunofluorescence - Anti-Slingshot homolog 1/SSH1 antibody (ab76943)ICC/IF image of ab76943 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
