Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [EMab-134] to EGFR - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR antibody [EMab-134] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Mouse monoclonal [EMab-134] to EGFR - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and MDA-MB-468 whole cell lysates. IHC-P: Human oral carcinoma and lung carcinoma tissue; Human skin tissue. Flow Cyt: A431 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264547 is the carrier-free version of ab264540. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    Western blot - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    All lanes : Anti-EGFR antibody [EMab-134] (ab264540) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Predicted band size: 134 kDa
    Observed band size: 130,180 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 15 secs; Lane 3: 8 secs.

    The molecular weight observed is in consistent with the literature (PMID: 9090976).
    Negative control: SW620 (PMID: 19789347).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab264540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling EGFR with ab264540 at 1/100 dilution followed by a ready to use secondary. Membranous staining on human skin is observed. The section was incubated with ab264540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab264540).

  • Flow Cytometry - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    Flow Cytometry - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)

    Flow cytometric analysis of SW620 (human colorectal adenocarcinoma epithelial cell, Left panel), A431 (human epidermoid carcinoma epithelial cell, Right panel) cells labelling EGFR with ab264540 at 1/100 dilution (1µg) (Red) compared with a mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: SW620.

    Gated on viable cells.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab264540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling EGFR with ab264540 at 1/100 dilution followed by a ready to use secondary. Membranous staining on human lung carcinoma tissue is observed. The section was incubated with ab264540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab264540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)

    Immunohistochemical analysis of paraffin-embedded human oral carcinoma tissue labeling EGFR with ab264540 at 1/100 dilution followed by a ready to use secondary. Membranous staining on human oral carcinoma tissue is observed. The section was incubated with ab264540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab264540).

  • Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)
    Anti-EGFR antibody [EMab-134] - BSA and Azide free (ab264547)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.