Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

Key features and details

  • Mouse monoclonal [T6] to Prolactin Receptor/PRL-R
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Prolactin Receptor/PRL-R antibody [T6]
    See all Prolactin Receptor/PRL-R primary antibodies

  • Description

    Mouse monoclonal [T6] to Prolactin Receptor/PRL-R

  • Host species

    Mouse

  • Specificity

    Detects Prolactin Receptor/PRL-R in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing Prolactin Receptor/PRL-R in NB2 cell lysate. Immunohistochemical staining of Prolactin Receptor/PRL-R in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to Prolactin Receptor/PRL-R in vitro.

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Full length native protein (purified) corresponding to Rat Prolactin Receptor/PRL-R. Purified rat liver PRL receptor.

  • Positive control

    • IHC-P: Rat pituitary gland tissue. ICC: C6, H-4-II-E, SW480 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

  • Clonality

    Monoclonal

  • Clone number

    T6

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
    Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

    Immunocytochemical analysis of C6 (Rat glial tumor cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
    Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

    Immunocytochemical analysis of H-4-II-E (Rat hepatoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
    Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

    Immunocytochemical analysis of in SW480 (Human colorectal adenocarcinoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

    Immunohistochemical analysis of normal biopsies of deparaffinized rat pituitary gland tissue labeling Prolactin Receptor/PRL-R. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

© 2021 Astana Biomed Group LLP. All rights reserved.