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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper

Alexa Fluor® 488 Anti-MiTF antibody [D5] (ab201675)

Price and availability

294 835 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Alexa Fluor® 488 Anti-MiTF antibody [D5] (ab201675)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [D5] to MiTF
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

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Overview

  • Product name

    Alexa Fluor® 488 Anti-MiTF antibody [D5]
    See all MiTF primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [D5] to MiTF
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, DogDoes not react with: Rat
  • Epitope

    N-terminal
  • Positive control

    • Flow Cyt: MALME-3M cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    D5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Immunology
    • Innate Immunity
    • Mast Cells

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-MiTF antibody [D5] (ab201675)
    Flow Cytometry - Alexa Fluor® 488 Anti-MiTF antibody [D5] (ab201675)

    Overlay histogram showing MALME-3M cells stained with ab201675 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab201675, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in MALME-3M cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Alexa Fluor® 488 Anti-MiTF antibody [D5] (ab201675)

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    Anti-MiTF antibody [SPM290] (ab233928)

    Applications: IHC-P

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    Anti-MiTF antibody [C5] (ab12039)

    Applications: Flow Cyt, WB

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    Anti-MiTF antibody (ab59232)

    Applications: ICC/IF, IHC-P

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    Anti-MiTF antibody [D5] (ab3201)

    Applications: Flow Cyt, ICC/IF, WB

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    Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)

    Applications: Flow Cyt, IP, WB

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    Anti-MiTF antibody [EPR9731] - BSA and Azide free (ab232415)

    Applications: WB

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    Anti-MiTF antibody [SPM290] - BSA and Azide free (ab212610)

    Applications: IHC-P

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    Anti-MiTF antibody [MITF/2987R] (ab270262)

    Applications: IHC-P

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    Anti-MiTF antibody [EPR9731] (ab140606)

    Applications: WB

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