Anti-MiTF antibody (ab20663)
Key features and details
- Rabbit polyclonal to MiTF
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-MiTF antibody
See all MiTF primary antibodies -
Description
Rabbit polyclonal to MiTF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC (PFA fixed) -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human MiTF.
Read Abcam's proprietary immunogen policy (Peptide available as ab21454.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab20663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Application notesIs unsuitable for IHC (PFA fixed).Target
-
Function
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. -
Tissue specificity
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. -
Involvement in disease
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
Sequence similarities
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4286 Human
- Entrez Gene: 17342 Mouse
- Entrez Gene: 25094 Rat
- Omim: 156845 Human
- SwissProt: O75030 Human
- SwissProt: Q08874 Mouse
- SwissProt: O88368 Rat
- Unigene: 166017 Human
see all -
Alternative names
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
Images
-
Western blot - Anti-MiTF antibody (ab20663)All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HeLa whole cell lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lane 5 : HeLa nuclear lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lane 6 : A431 whole cell lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 25 kDa, 37 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-MiTF antibody (ab20663)All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Protocols
References (20)
ab20663 has been referenced in 20 publications.
- Sun L et al. Syntenin regulates melanogenesis via the p38 MAPK pathway. Mol Med Rep 22:733-738 (2020). PubMed: 32626944
- Li X et al. Cyclin-dependent kinase 5 regulates proliferation, migration, tyrosinase activity, and melanin production in B16-F10 melanoma cells via the essential regulator p-CREB. In Vitro Cell Dev Biol Anim 55:416-425 (2019). PubMed: 31069610
- Miao F et al. Intramelanocytic Acidification Plays a Role in the Antimelanogenic and Antioxidative Properties of Vitamin C and Its Derivatives. Oxid Med Cell Longev 2019:2084805 (2019). PubMed: 31214276
- Qi S et al. Knockdown of microRNA-143-5p by STTM technology affects eumelanin and pheomelanin production in melanocytes. Mol Med Rep 20:2649-2656 (2019). PubMed: 31322203
- Qian L et al. Downregulation of microRNA-218 is cardioprotective against cardiac fibrosis and cardiac function impairment in myocardial infarction by binding to MITF. Aging (Albany NY) 11:5368-5388 (2019). PubMed: 31408435
Images
-
Western blot - Anti-MiTF antibody (ab20663)All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HeLa whole cell lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lane 5 : HeLa nuclear lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lane 6 : A431 whole cell lysate withHuman MiTF peptide (ab21454) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 25 kDa, 37 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-MiTF antibody (ab20663)All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
