Recombinant human CD80 protein (Active) (ab173993)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, HPLC, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 10% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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SDS-PAGE - Recombinant human CD80 protein (ab173993)
Reduced SDS-PAGE analysis of ab173993, stained overnight with Coomassie Blue. The protein migrates as 65-90 kDa due to glycosylation.
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HPLC - Recombinant human CD80 protein (ab173993)The purity of ab173993 was greater than 95% as determined by SEC-HPLC.
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ELISA - Recombinant human CD80 protein (ab173993)Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 (biotinylated) protein (ab207136) with a linear range of 0.6-10 ng/mL.
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Functional Studies - Recombinant human CD80 protein (ab173993)FACS assay shows that ab173993 can bind to 293T cells overexpressing human CTLA4 protein. The concentration of ab173993 is 1 μg/mL.
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Functional Studies - Recombinant human CD80 protein (ab173993)Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (ab246118) with a linear range of 0.02-1.25 μg/mL
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Functional Studies - Recombinant human CD80 protein (ab173993)Immobilized Human CD28, on CM5 Chip can bind ab173993 with an affinity constant of 2.01 μM as determined in a SPR assay.
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ELISA - Recombinant human CD80 protein (ab173993)Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA4 protein (ab167727) with a linear range of 0.5-4 ng/mL.
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ELISA - Recombinant human CD80 protein (ab173993)Immobilized Biotinylated Mouse CD28, His, Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ab173993 with a linear range of 0.2-2 ng/mL.
