Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)
![Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)](https://www.abcam.com/ps/products/264/ab264110/Images/ab264110-355596-AntiMiTF-antibody-C5--ChIP-Grade-flow-cytometry-malme3m-human.jpg "Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)")
Key features and details
- Mouse monoclonal [C5] to MiTF - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MiTF antibody [C5] - BSA and Azide free
See all MiTF primary antibodies -
Description
Mouse monoclonal [C5] to MiTF - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment corresponding to Human MiTF (N terminal).
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Positive control
- WB: Malme-3M whole cell lysate. Flow Cyt: Malme-3M cells.
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General notes
ab264110 is the PBS only version of ab12039.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
C5 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)
This image was produced using the same antibody clone, but in a different formulation, ab12039.
Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
