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Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)

Key features and details

  • Mouse monoclonal [C5] to MiTF - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-MiTF antibody [C5] - BSA and Azide free
    See all MiTF primary antibodies

  • Description

    Mouse monoclonal [C5] to MiTF - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human MiTF (N terminal).

  • Positive control

    • WB: Malme-3M whole cell lysate. Flow Cyt: Malme-3M cells.

  • General notes

    ab264110 is the PBS only version of ab12039.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Flow Cytometry - Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)
    Flow Cytometry - Anti-MiTF antibody [C5] - BSA and Azide free (ab264110)

    This image was produced using the same antibody clone, but in a different formulation, ab12039.

    Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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