HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
![HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)](https://www.abcam.com/ps/products/185/ab185058/Images/ab185058-221381-anti-muscle-actin-antibody-epr8484-loading-control-hrp-western-blot.jpg "HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR8484] to muscle Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-muscle Actin antibody [EPR8484] - Loading Control
See all muscle Actin primary antibodies -
Description
HRP Rabbit monoclonal [EPR8484] to muscle Actin - Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide within Human muscle Actin aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P68032 -
Positive control
- NIH 3T3 cell lysate; Human fetal artery, kidney and heart lisates; Human uterus, stomach and skeletal muscle lysates; Human skeletal muscle tissue; Human smooth muscle in colon tissue; Human heart muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8484 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)All lanes : HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058) at 1/10000 dilution
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 2 : Human kidney tissue lysate - total protein (ab30203) at 20 µg
Lane 3 : Human Fetal Heart at 5 µg
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Lane 5 : Skeletal Muscle (Rat) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185058 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406
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![HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)](https://www.abcam.com/ps/products/185/ab185058/Images/ab185058-2-benefits-of-recombinant-antibodies.png)
HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
