Anti-muscle Actin antibody [HHF35] (ab7813)
![Anti-muscle Actin antibody [HHF35] (ab7813)](https://www.abcam.com/ps/products/7/ab7813/Images/ab7813-287887-anti-muscle-actin-antibody-hhf35-immunohistochemistry.jpg "Anti-muscle Actin antibody [HHF35] (ab7813)")
Key features and details
- Mouse monoclonal [HHF35] to muscle Actin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-muscle Actin antibody [HHF35]
See all muscle Actin primary antibodies -
Description
Mouse monoclonal [HHF35] to muscle Actin -
Host species
Mouse -
Specificity
This antibody recognizes muscle specific alpha and gamma actin isomers but is non-reactive with beta isomers. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
The SDS extracted protein fraction of human myocardium.
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General notes
This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative. -
Clonality
Monoclonal -
Clone number
HHF35 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] (ab7813)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling muscle Actin with ab7813.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] (ab7813)](https://www.abcam.com/ps/products/7/ab7813/Images/ab7813-13282-anti-muscle-actin-antibody-hhf35-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] (ab7813)Ab7813 staining human normal skeletal muscle. Staining is localized to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
