Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19352] to MAX - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MAX antibody [EPR19352] - BSA and Azide free
    See all MAX primary antibodies

  • Description

    Rabbit monoclonal [EPR19352] to MAX - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab236028 reacted weakly in tissue lysate samples and requires further optimization.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, Jurkat, K562 and Raji whole cell lysates.

  • General notes

    Ab236028 is the carrier-free version of ab199489. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236028 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)
    Immunoprecipitation - Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)

    MAX was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab199489 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab199489 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: 293 whole cell lysate 10µg (Input).

    Lane 2: ab199489 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199489 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199489).

  • Western blot - Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)
    Western blot - Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)
    All lanes : Anti-MAX antibody [EPR19352] (ab199489) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199489).

  • Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)
    Anti-MAX antibody [EPR19352] - BSA and Azide free (ab236028)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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