Anti-MAX antibody [73C5a] (ab53570)
![Anti-MAX antibody [73C5a] (ab53570)](https://www.abcam.com/ps/products/53/ab53570/Images/ab53570-66444-anti-max-antibody-73c5a-western-blot.gif "Anti-MAX antibody [73C5a] (ab53570)")
Key features and details
- Mouse monoclonal [73C5a] to MAX
- Suitable for: Flow Cyt, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-MAX antibody [73C5a]
See all MAX primary antibodies -
Description
Mouse monoclonal [73C5a] to MAX -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant human MAX
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
73C5a -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-MAX antibody [73C5a] (ab53570)Anti-MAX antibody [73C5a] (ab53570) + immunised recombinant protein
Predicted band size: 18 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)](https://www.abcam.com/ps/products/53/ab53570/Images/ab53570-66443-anti-max-antibody-73c5a-flow-cytometry.jpg)
Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
