Anti-c-Myc (phospho T58) antibody (ab28842)
Key features and details
- Rabbit polyclonal to c-Myc (phospho T58)
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Myc (phospho T58) antibody
See all c-Myc primary antibodies -
Description
Rabbit polyclonal to c-Myc (phospho T58) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human c-Myc aa 25-74. The exact sequence is proprietary.
Database link: P01106 -
Positive control
- Purchase matching WB positive control:Recombinant Human c-Myc protein
- Ovarian cancer cell lysate, breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Myc (phospho T58) antibody (ab28842)Lane 1 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution
Lane 2 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized non-
phosphopeptide
)
Lane 3 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized
phosphopeptide
)
Lanes 1 & 3 : human ovarian cancer cell lysate
Lane 2 : human ovarian cancer cell lysate
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 55 kDa, 86 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody (ab28842)ICC/IF image of ab28864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho T58) antibody (ab28842)Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Myc (Phospho-Thr58) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
