Anti-c-Myc (phospho S62) antibody (ab51156)
Key features and details
- Rabbit polyclonal to c-Myc (phospho S62)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Myc (phospho S62) antibody
See all c-Myc primary antibodies -
Description
Rabbit polyclonal to c-Myc (phospho S62) -
Host species
Rabbit -
Specificity
ab51156 detects endogenous levels of Myc only when phosphorylated at serine 62. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human c-Myc (N terminal) (phospho S62). The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of serine 62 (P-L-SP-P-S).
Database link: P01106 -
Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Myc (phospho S62) antibody (ab51156)All lanes : Anti-c-Myc (phospho S62) antibody (ab51156) at 1/500 dilution
Lane 1 : extracts from 293 cells treated
with 40nM Forskolin for 30min, with phosphopeptide
Lane 2 : extracts from 293 cells treated with 40nM Forskolin for 30min, without phosphopeptide
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody (ab51156)Immunohistochemical analysis of c-Myc (phospho S62) expression in paraffin-embedded human breast carcinoma tissue using 1/50 ab51156. left: untreated sample. Right: sample treated with phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (ab51156)Immunofluorescence analysis of HeLa cells, treated (left) and untreated (right) with Forskolin (40nM, 15mins), using c-Myc (phospho-Ser62) antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (ab51156)ICC/IF image of ab51156 stained HepG2 cells. The cells were 4% formlaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
