Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SM3] to MUC1
Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-MUC1 antibody [SM3]
See all MUC1 primary antibodies
Description

Rabbit monoclonal [SM3] to MUC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Full length native protein (purified) corresponding to MUC1. Hydrogen fluoride deglycosylated milk mucin.
Positive control
- WB: MCF7 cell lysate. Flow Cyt: MCF7 cells. IHC-P: Human lung tissue. ICC/IF: MCF7 cells.
General notes

Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Proclin 300
Constituent: 99% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

SM3
Isotype

IgG
Light chain type

lambda
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [SM3] (ab245695)

PFA fixed, 0.15% Triton permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling MUC1 using ab245695 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor^® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [SM3] (ab245695)

Paraffin-embedded human lung tissue stained for MUC1 with ab245695 at 4 µg/ml, 30 mins in immunohistochemical analysis. Antigen retreival was acheived by microwaving in citrate buffer (pH 6), followed by blocking with protein block serum-free buffer. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3′-diaminobenzidine), and counterstaining with hemotoxylin.
Flow Cytometry - Anti-MUC1 antibody [SM3] (ab245695)

MCF7 (Human breast adenocarcinoma cell line) cells were stained with unimmunized rabbit IgG antibody (black line) or ab245695 (blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 µg/ml.
Western blot - Anti-MUC1 antibody [SM3] (ab245695)

Anti-MUC1 antibody [SM3] (ab245695) at 1 µg/ml (incubated for 1 hr) + MCF7 (Human breast adenocarcinoma cell line) cell lysate (RIPA buffer) at 35 µg

Predicted band size: 122 kDa

10% SDS PAGE gel.

Detected by chemiluminescence.
Anti-MUC1 antibody [SM3] (ab245695)

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