Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1024Y] to MUC1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-Fr, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MUC1 antibody [EP1024Y] - BSA and Azide free
    See all MUC1 primary antibodies

  • Description

    Rabbit monoclonal [EP1024Y] to MUC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Fr, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • T47D, MCF7 and A549 cell lysates, human kidney, human breast carcinoma, human thyroid carcinoma, human colon cancer lysate, human fetal lung lysate, rat liver lysate and mouse liver lysate.

  • General notes

    ab256588 is the carrier-free version of ab45167. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256588 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)
    Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling MUC1 with purified ab45167 at 1/500 dilution (0.2μg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, a goat anti-rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as counterstain at 1/200 dilution (2.5 μg/ml). The negative control is PBS instead of the primary antibody. Nuclei counterstained with DAPI (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45167).

  • Flow Cytometry - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)
    Flow Cytometry - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)

    Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) cells labelling MUC1 with unpurified ab45167 (pink) at a dilution of 1/150. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45167).

  • Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)
    Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)

    ab45167 (purified) at 1/20 dilution (2ug) immunoprecipitating MUC1 in Human fetal lung lysate.

    Lane 1 (input): Human fetal lung lysate 10ug

    Lane 2 (+): ab45167 + Human fetal lung lysate 10ug

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45167 in Human fetal lung lysate

    For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45167).

  • Flow Cytometry - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)
    Flow Cytometry - Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)

    Flow Cytometry analysis of A549 (human lung carcinoma cell line) cells labeling MUC1 with purified ab45167 at 1/20 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabeled control, cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45167).

  • Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)
    Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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