Anti-MUC1 antibody [EPR1025(2)] (ab181133)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1025(2)] to MUC1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MUC1 antibody [EPR1025(2)]
See all MUC1 primary antibodies -
Description
Rabbit monoclonal [EPR1025(2)] to MUC1 -
Host species
Rabbit -
Specificity
Based on the blast result, this antibody only recognizes isoform 5 of MUC1(Mucin-1). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SKBR-3, MCF-7, T47-D and SW480 cell lysates; IHC-P: Human colon, thyroid and cervical carcinoma tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1025(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling MUC1 with Purified ab181133 at 1:3000 dilution (0.22 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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Anti-MUC1 antibody [EPR1025(2)] (ab181133) at 0.1 µg/ml (purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 104 kDaBlocking and diluting buffer: 5% NFDM/TBST
The observed band is consistent with the papers (PMID: 25757505 and 10197628) -
All lanes : Anti-MUC1 antibody [EPR1025(2)] (ab181133) at 1/1000 dilution
Lane 1 : SKBR-3 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : T47-D cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 104 kDa
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MUC1 with ab181133 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling MUC1 with ab181133 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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