Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR2DL3 antibody [EPR22192] (ab225740)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22192] to KIR2DL3
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR2DL3 antibody [EPR22192]
    See all KIR2DL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22192] to KIR2DL3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: transfected HEK-293T cells IHC: human endometrium and tonsil tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22192

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 antibody [EPR22192] (ab225740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 antibody [EPR22192] (ab225740)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL3 with ab225740 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID: 19628706). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 antibody [EPR22192] (ab225740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 antibody [EPR22192] (ab225740)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling KIR2DL3 with ab225740 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in natural killer (NK) cells in marginal zone of human tonsil (PMID: 19628706). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-KIR2DL3 antibody [EPR22192] (ab225740)
    Western blot - Anti-KIR2DL3 antibody [EPR22192] (ab225740)
    All lanes : Anti-KIR2DL3 antibody [EPR22192] (ab225740) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with KIR2DL3 (WT) expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50-60 kDa
    why is the actual band size different from the predicted?



    Exposure time : 5.5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-KIR2DL3 antibody [EPR22192] (ab225740)
    Anti-KIR2DL3 antibody [EPR22192] (ab225740)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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