Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)
![Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)](https://www.abcam.com/ps/products/28/ab28081/Images/ab28081-38971-anti-muc1-antibody-c595-ncrc48-immunohistochemistry.jpg "Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)")
Key features and details
- Mouse monoclonal [C595 (NCRC48)] to MUC1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-MUC1 antibody [C595 (NCRC48)]
See all MUC1 primary antibodies -
Description
Mouse monoclonal [C595 (NCRC48)] to MUC1 -
Host species
Mouse -
Specificity
ab28081 recognises the breast cancer associated mucin encoded by the Muc-1 gene, CD227. In normal tissues expression is restricted to specialised glandular epithelial cells. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Urinary MUC-1 mucin (Human)
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Epitope
ab28081 recognises the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. -
Positive control
- Breast carcinoma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
C595 (NCRC48) -
Isotype
IgG3 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)Ab28081 staining human normal lung. Staining is localised to the apical cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir -
![Western blot - Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)](https://www.abcam.com/ps/products/28/ab28081/Images/ab28081-38972-anti-muc1-antibody-c595-ncrc48-western-blot.jpg)
Western blot - Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)Anti-MUC1 antibody [C595 (NCRC48)] (ab28081) + Whole cell lysate prepared from human BT20 cells
Predicted band size: 122 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)](https://www.abcam.com/ps/products/28/ab28081/Images/ab28081-38970-anti-muc1-antibody-c595-ncrc48-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)FITC-conjugated ab28081 staining MUC1 in MCF-7 cells (green). Nuclei are counterstained with DAPI (blue).
