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PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab215670)

PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab215670)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • PE Mouse monoclonal [HMFG1 (aka 1.10.F3)] to MUC1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

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Overview

  • Product name

    PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
    See all MUC1 primary antibodies
  • Description

    PE Mouse monoclonal [HMFG1 (aka 1.10.F3)] to MUC1
  • Host species

    Mouse
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    Other Immunogen Type corresponding to Human MUC1. (Delipidated human milk fat globule).

  • Positive control

    • Flow Cyt: A431 cells.
  • General notes

    For a more comprehensive guide to this epitope of HMFG1 clone, we recommend the following publications;

    Petrakou E et al. 1998: Epitope Mapping of Anti-MUC1 Mucin Protein Core Monoclonal Antibodies. Tumour Biology.

    Verhoeyen ME et al. 1993: Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody. Immunology.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    HMFG1 (aka 1.10.F3)
  • Myeloma

    P3-NS1/1-Ag4-1
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Images

  • Flow Cytometry - PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab215670)
    Flow Cytometry - PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab215670)

    Overlay histogram showing A431 cells stained with ab215670 (red line). The cells were fixed with 80% Methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215670, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 [B11/6] (monoclonal) Phycoerythrin (ab91357) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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