Anti-APC2 antibody [3A2G2] (ab233753)
![Anti-APC2 antibody [3A2G2] (ab233753)](https://www.abcam.com/ps/products/233/ab233753/Images/ab233753-307317-anti-adenomatous-polyposis-coli-protein-2-antibody-3a2g2-western-blot.jpg "Anti-APC2 antibody [3A2G2] (ab233753)")
Key features and details
- Mouse monoclonal [3A2G2] to APC2
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-APC2 antibody [3A2G2]
See all APC2 primary antibodies -
Description
Mouse monoclonal [3A2G2] to APC2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human APC2 aa 2041-2181. (Expressed in E.coli).
Database link: O95996 -
Positive control
- WB: Human APC2 (aa 2041-2181) recombinant protein; APC2 (aa 2041-2181)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells. IHC-P: Human ovarian cancer and bladder cancer tissues.
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General notes
This product was previously labelled as Adenomatous polyposis coli protein 2
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A2G2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-APC2 antibody [3A2G2] (ab233753)Anti-APC2 antibody [3A2G2] (ab233753) at 1/500 dilution + Human APC2 (aa 2041-2181) recombinant protein
Predicted band size: 244 kDaExpected MW is 40.9 kDa.
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![Western blot - Anti-APC2 antibody [3A2G2] (ab233753)](https://www.abcam.com/ps/products/233/ab233753/Images/ab233753-307316-anti-adenomatous-polyposis-coli-protein-2-antibody-3a2g2-western-blot.jpg)
Western blot - Anti-APC2 antibody [3A2G2] (ab233753)All lanes : Anti-APC2 antibody [3A2G2] (ab233753) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : APC2 (aa 2041-2181)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 244 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-APC2 antibody [3A2G2] (ab233753)](https://www.abcam.com/ps/products/233/ab233753/Images/ab233753-307314-anti-adenomatous-polyposis-coli-protein-2-antibody-3a2g2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-APC2 antibody [3A2G2] (ab233753)HeLa (human prostate adenocarcinoma cell line) cells stained for APC2 (green) using ab233753 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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![Flow Cytometry - Anti-APC2 antibody [3A2G2] (ab233753)](https://www.abcam.com/ps/products/233/ab233753/Images/ab233753-307313-anti-adenomatous-polyposis-coli-protein-2-antibody-3a2g2-flow-cytometry.jpg)
Flow Cytometry - Anti-APC2 antibody [3A2G2] (ab233753)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labelling APC2 with ab233753 at 1/200 dilution (green) compared with a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC2 antibody [3A2G2] (ab233753)](https://www.abcam.com/ps/products/233/ab233753/Images/ab233753-307312-anti-adenomatous-polyposis-coli-protein-2-antibody-3a2g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC2 antibody [3A2G2] (ab233753)Paraffin-embedded human ovarian cancer tissue stained for APC2 using ab233753 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC2 antibody [3A2G2] (ab233753)](https://www.abcam.com/ps/products/233/ab233753/Images/ab233753-307311-anti-adenomatous-polyposis-coli-protein-2-antibody-3a2g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC2 antibody [3A2G2] (ab233753)Paraffin-embedded human bladder cancer tissue stained for APC2 using ab233753 at 1/200 dilution in immunohistochemical analysis. DAB staining.
