Anti-CD163 antibody [RM3/1] (ab17051)
![Anti-CD163 antibody [RM3/1] (ab17051)](https://www.abcam.com/ps/products/17/ab17051/Images/ab17051-454447-anti-cd163-antibody-rm3-1-immumnohistochemistry-frozen-tonsil-human.jpg "Anti-CD163 antibody [RM3/1] (ab17051)")
Key features and details
- Mouse monoclonal [RM3/1] to CD163
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD163 antibody [RM3/1]
See all CD163 primary antibodies -
Description
Mouse monoclonal [RM3/1] to CD163 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details
Unsuitable for: IHC-G or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Rat, Guinea pig, Pig -
Immunogen
Human monocytes (epitope recognized SRCR domain 9).
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Positive control
- Flow Cyt: THP-1 cells. IHC-Fr: Human tonsil tissue.
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General notes
ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purification notes
0.2 µm filtered. -
Primary antibody notes
ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment. -
Clonality
Monoclonal -
Clone number
RM3/1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-CD163 antibody [RM3/1] (ab17051)
Immunohistochemistry analysis of frozen human tonsil tissue sections (interfollicular) stained for CD163 using ab17051 at a 1/10 dilution.
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![Flow Cytometry - Anti-CD163 antibody [RM3/1] (ab17051)](https://www.abcam.com/ps/products/17/ab17051/Images/ab17051-314094-anti-cd163-antibody-rm3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-CD163 antibody [RM3/1] (ab17051)Flow cytometric detection of CD163 in THP-1 cells. The black line represents cells only, the red line the isotype control and the blue line ab17051 in a concentration of 2 μg/250000 cells.
