Feline IFN gamma ELISA Kit (ab273229)
Key features and details
- Sensitivity: 0.24 ng/ml
- Range: 0.24 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Feline IFN gamma ELISA Kit
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.24 ng/ml -
Range
0.24 ng/ml - 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 78.81 74% - 88% Serum 94.51 68% - 118% Plasma 90.2 75% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Feline IFN gamma ELISA Kit (ab273229) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IFN gamma in serum (feline IFN gamma concentration is low in normal serum/ plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for feline IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- feline IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Feline IFN gamma coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Feline IFN gamma Antibody 2 vials Feline IFN gamma Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 493965 Cat
- SwissProt: P46402 Cat
Images
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Example standard curve