Mouse Limitin ELISA Kit (ab267588)
Key features and details
- Sensitivity: 6.2 pg/ml
- Range: 6.14 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Limitin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.2 pg/ml -
Range
6.14 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 125.6 112% - 144% Plasma 125.3 97% - 147% Cell culture media 117.9 92% - 147% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Limitin ELISA Kit is designed for the quantitative determination of Limitin in cell culture supernatants, plasma and serum samples.
Δ Note: Mouse Limitin concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for mouse Limitin coated on a 96-well plate. Standards and samples are pipetted into the wells and Limitin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Limitin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Limitin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BAFF R, Betacellulin (BTC), C5a, CCL6, CD48 (SLAMF2), CD6, CD80 (B7-1), Chemerin, Clusterin, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33 (IL-1 F11), IL-7 R alpha, Kremen-1, Lipocalin-2 (NGAL), LOX-1, Lungkine (CXCL15), Marapsin, MBL-2, Meteorin, Nope, NOV (CCN3), Osteoactivin (GPNMB), OX40 Ligand, P-Cadherin, Periostin, PlGF 2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1 (KIM-1), TRAIL (TNFSF10), Tryptase epsilon.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Mouse Limitin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse Limitin Detection Antibody 2 vials Mouse Limitin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
Produced by macrophages, IFN alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase -
Cellular localization
Secreted -
Alternative names
- IFN alpha 11
- Ifna11
- Interferon alpha 11
- Limitin
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Database links
- Entrez Gene: 15964 Mouse
- SwissProt: Q61716 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.