Rat CCL3 ELISA Kit (ab242242)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.96 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CCL3 ELISA Kit
See all Macrophage Inflammatory Protein 1 alpha / CCL3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 3 3.5% Inter-assay Sample n Mean SD CV% supernatant 8 3.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.96 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 91 90% - 93% Serum 84 82% - 85% Hep Plasma 85 81% - 89% EDTA Plasma 75 72% - 78% Cit plasma 78 77% - 78% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat CCL3 ELISA Kit (ab242242) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CCL3 with 1.96 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CCL3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat CCL3 Capture Antibody (Lyophilized) 1 vial Rat CCL3 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine 3
- CCL 3
- CCL3
see all -
Database links
- Entrez Gene: 25542 Rat
- SwissProt: P50229 Rat
- Unigene: 10139 Rat
Images
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Other - Rat CCL3 ELISA Kit (ab242242)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat CCL3 standard curve in Sample Diluent NS.The CCL3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of spiked CCL3 in rat serum and plasma samples.The concentrations of CCL3 were measured in duplicates, interpolated from the CCL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, plasma (EDTA) 50% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentrations of native CCL3 in stimulated and unstimulated rat PBMC supernatant samples.The concentrations of CCL3 were measured in duplicates, interpolated from the CCL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated rat PBMC supernatant 5% and unstimulated rat PBMC supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL3 concentration was determined to be 5,805 pg/mL in stimulated rat PBMC supernatant and was undetectable in unstimulated rat PBMC supernatant. -
Sandwich ELISA - Rat CCL3 ELISA Kit (ab242242)To learn more about the advantages of recombinant antibodies see here.
