Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSG6 antibody [EPR17582-45] (ab267469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17582-45] to TSG6
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-TSG6 antibody [EPR17582-45]
    See all TSG6 primary antibodies

  • Description

    Rabbit monoclonal [EPR17582-45] to TSG6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours,) whole cell lysate; Rat amniotic membrane tissue lysate. Flow cyt: THP-1 (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) cells. IP: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Western blot - Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Anti-TSG6 antibody [EPR17582-45] (ab267469) at 1/1000 dilution + Rat amniotic membrane tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 31 kDa
    Observed band size: 120,36 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).

    The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).

    Exposure time: 3 minutes

     

  • Flow Cytometry - Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Flow Cytometry - Anti-TSG6 antibody [EPR17582-45] (ab267469)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) (Red) / Untreated control (Green) cells labelling TSG6 with ab267469 at 1/500 dilution (0.1ug) (Red, Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] (ab267469)

    TSG6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate with ab267469 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267469 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate 10 ug.

    Lane 2: ab267469 IP in THP-1 (treated as above).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267469 in THP-1 (treated as above) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Western blot - Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Lane 1 : Anti-TSG6 antibody [EPR17582-45] (ab267469) at 1/1000 dilution
    Lanes 2-3 : Anti-p75 NGF Receptor antibody [NORI138B] (ab271289) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours), whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 120,36 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).

    The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).

    Negative control: A549 (PMID: 19389798).

    Exposure time: 3 minutes

     

  • Anti-TSG6 antibody [EPR17582-45] (ab267469)
    Anti-TSG6 antibody [EPR17582-45] (ab267469)

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