Human Prekallikrein 1B ELISA Kit, Fluorescent (ab229411)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 76 pg/ml
- Range: 0.12 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Prekallikrein 1B ELISA Kit, Fluorescent
See all Plasma Kallikrein 1B kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 6.3% Inter-assay Sample n Mean SD CV% Serum 3 5.8% -
Sample type
Cell culture supernatant, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
76 pg/ml -
Range
0.12 ng/ml - 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 87% - 101% Milk 85 82% - 91% Serum 92 84% - 98% Hep Plasma 91 87% - 97% EDTA Plasma 101 88% - 107% Cit plasma 92 87% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Sheep, Goat, Cow, Pig -
Product overview
Prekallikrein 1B in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Plasma Kallikrein 1B protein in human serum, plasma, milk, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
Prekallikrein 1B (KLKB1) is a 88 kDa serine protease involved in blood coagulation, kinin generation, fibrinolysis and inflammation. Mature KLKB1 primarily circulates as a complex with high-molecular weight kinnogen, where it releases kinins from kininogens during the inflammation and vasodilation response.
This kit detects both prekallikrein and plasma kallikrein.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Prekallikrein 1B Capture Antibody 1 x 600µl 10X Human Prekallikrein 1B Detector Antibody 1 x 600µl Human Prekallikrein 1B Lyophilized Purified Protein 2 vials Antibody Diluent 4BI 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml Stoplight Red Substrate Buffer 1 x 12ml 100X Stoplight Red Substrate 1 x 120µl 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Plate Seals 1 unit -
Research areas
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Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. -
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Fletcher factor
- kallikrein B plasma
- Kallikrein B plasma (Fletcher factor) 1
see all -
Database links
- Entrez Gene: 3818 Human
- Omim: 229000 Human
- SwissProt: P03952 Human
- Unigene: 237642 Human
Images
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Other - Human Prekallikrein 1B ELISA Kit, Fluorescent (ab229411)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Prekallikrein 1B standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of human serum and plasma within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Interpolated concentrations of Prekallikrein 1B levels in individual healthy donors.Serum from 10 healthy male donors was measured in duplicate. The mean Plasma Kallikrein 1B concentration was determined to be 112.6 µg/mL with a range of 80.8 – 206.4 µg/mL in male donors.
