Anti-Timeless antibody [EPR5274(2)] (ab133569)
![Anti-Timeless antibody [EPR5274(2)] (ab133569)](https://www.abcam.com/ps/products/133/ab133569/Images/ab133569-147341-anti-timeless-antibody-epr52742-flow-cytometry.jpg "Anti-Timeless antibody [EPR5274(2)] (ab133569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5274(2)] to Timeless
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Timeless antibody [EPR5274(2)]
See all Timeless primary antibodies -
Description
Rabbit monoclonal [EPR5274(2)] to Timeless -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseWB Human
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Immunogen
Synthetic peptide within Human Timeless aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UNS1 -
Positive control
- NIH/3T3 mouse cells; Jurkat, HeLa, JAR, RAW 264.7, PC12 and NIH/3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5274(2) -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Timeless antibody [EPR5274(2)] (ab133569)Flow cytometry analysis of permeabilized mouse NIH/3T3 cells staining Timeless using ab133569 at a 1/10 dilution (red) or a rabbit IgG control antibody (green).
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![Western blot - Anti-Timeless antibody [EPR5274(2)] (ab133569)](https://www.abcam.com/ps/products/133/ab133569/Images/ab133569-147340-anti-timeless-antibody-epr52742-western-blot.jpg)
Western blot - Anti-Timeless antibody [EPR5274(2)] (ab133569)All lanes : Anti-Timeless antibody [EPR5274(2)] (ab133569) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 139 kDa
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![Anti-Timeless antibody [EPR5274(2)] (ab133569)](https://www.abcam.com/ps/products/133/ab133569/Images/ab133569-3-benefits-of-recombinant-antibodies.png)
Anti-Timeless antibody [EPR5274(2)] (ab133569)
