Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5275] to Timeless - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Timeless antibody [EPR5275] - BSA and Azide free
    See all Timeless primary antibodies

  • Description

    Rabbit monoclonal [EPR5275] to Timeless - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239982 is the carrier-free version of ab109512 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239982 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)
    Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling Timeless with Purified ab109512 at 1/1000 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109512).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109512 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109512).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109512 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109512).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)
    Anti-Timeless antibody [EPR5275] - BSA and Azide free (ab239982)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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