Anti-Timeless antibody (ab229218)
Key features and details
- Rabbit polyclonal to Timeless
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Timeless antibody
See all Timeless primary antibodies -
Description
Rabbit polyclonal to Timeless -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Timeless (C terminal). The exact sequence is proprietary.
Database link: Q9UNS1 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates; Neuro-2a, C8D30, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. IP: HEK-293T whole cell extract. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Timeless antibody (ab229218)All lanes : Anti-Timeless antibody (ab229218) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 139 kDa5% SDS-PAGE gel.
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Western blot - Anti-Timeless antibody (ab229218)All lanes : Anti-Timeless antibody (ab229218) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 139 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody (ab229218)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Timeless (green) using ab229218 at 1/2000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunoprecipitation - Anti-Timeless antibody (ab229218)Timeless was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229218. Western blot was performed from the immunoprecipitate using ab229218. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab229218 in HEK-293T whole cell extract.
Lane 3: ab229218 IP in HEK-293T whole cell extract.
